IS26-Mediated Precise Excision of the IS26-aphA1a Translocatable Unit
ABSTRACT We recently showed that, in the absence of RecA-dependent homologous recombination, the Tnp26 transposase catalyzes cointegrate formation via a conservative reaction between two preexisting IS26, and this is strongly preferred over replicative transposition to a new site.Here, the reverse reaction was investigated by assaying for precise e